Protein Biology
How to Read a Western Blot: Bands, Sizes, and Common Surprises
A western blot tells a story — band size, intensity, controls, and unexpected bands all carry meaning.
A western blot tells a story — band size, intensity, controls, and unexpected bands all carry meaning.
Bradford is fast but detergent-sensitive. BCA tolerates detergents but reacts with reducing agents. Here’s how to pick the right one.
Protein purification follows a clear three-stage strategy: capture, intermediate purification, polishing.
A reliable western blot is 80% sample prep and 20% everything else. Here’s the protocol that consistently produces clean, publication-quality results.
SDS-PAGE looks simple until your bands are smeared, smiling, or invisible. Here’s a clean protocol plus fixes for every common failure mode.